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Indoor and Built Environment, Vol. 10, No. 3-4, 258-260 (2001)
DOI: 10.1177/1420326X0101000321

An Investigation of the Genotoxic Effects of Airborne Particulate Matter Using Single-Cell Gel Electrophoresis

Alison Curnow

Cornwall Dermatology Research Project, Royal Cornwall Hospital, Truro

Barbara Parsons

Cornwall College, Pool, Cornwall, UK

Leo Salter

Cornwall College, Pool, Cornwall, UK

Nick Morley

Cornwall Dermatology Research Project, Royal Cornwall Hospital, Truro

David Gould

Cornwall Dermatology Research Project, Royal Cornwall Hospital, Truro

Samples of the PM 10 fraction of airborne particulate mat ter were collected from a roadside location in Cornwall using a Partisol 2000 gravimetric air sampler. Cultured human lung fibroblasts were exposed to a sonicated extract of this matter for various periods of time (0-120 h) and the presence of DNA damage was then assessed using single-cell gel electrophoresis. Four times the level of DNA damage was detected in the PM10-exposed cells than that observed in similarly treated but non-PM10 exposed control cells. This indicates that the airborne PM10 from this site has significant genotoxic effects in this experimental system and that single-cell gel electro phoresis is a useful technique for the assessment of this form of particulate matter-induced biological damage.

Key Words: Comet ASSAY • DNA damage • Genotoxic effects • Particulate matter (PM10) • Single-cell gel electrophoresis


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