Indoor and Built Environment

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to register today!

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Culea, M.
Right arrow Articles by Podea, R.
Right arrow Search for Related Content
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Indoor and Built Environment, Vol. 14, No. 3-4, 293-299 (2005)
DOI: 10.1177/1420326X05054296
© 2005 International Society of the Built Environment

Exposure Assessment of Nicotine and Cotinine by GC-MS

Monica Culea

Babes-Bolyai University, Kogalniceanu str. No. 1, 3400 Cluj-Napoca, Romania, mculea{at}phys.ubbcluj.ro

Onuc Cozar

Babes-Bolyai University, Kogalniceanu str. No. 1, 3400 Cluj-Napoca, Romania

Simona Nicoara

Technical University of Cluj-Napoca, Romania

Razvan Podea

Babes-Bolyai University, Kogalniceanu str. No. 1, 3400 Cluj-Napoca, Romania

Environmental tobacco smoke exposure of human subjects in different public houses was evaluated. The levels of airborne nicotine from environmental tobacco smoke and changes in urinary cotinine and nicotine levels between smoking and non-smoking subjects were compared. A gas chromatography-mass spectrometric (GC-MS) quantitative method for nicotine and cotinine levels in the indoor air and in urine was developed. A Thermo Quest Trace DSQ GC-MS equipped with a Rtx-5MS capillary column, 15m 0.25mm, 0.25 m film thickness, was used in a temperature programme starting from 50°C, for 2min, then increased at 8°C·min-1 to 250°C and held for 10min. The method was validated and gave good linearity, precision, accuracy and limit of detection. A significant correlation between urinary cotinine increase and airborne nicotine is presented.

Key Words: Nicotine • Cotinine • GC-MS • Urine • ETS


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?