This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Pancer, K. Articles by Stypulkowska-Misiurewicz, H. Search for Related Content Social Bookmarking                         What's this?

Strains of Enterobacter cloacae Isolated in a Hospital Environment – Some Phenotypic and Genotypic Properties

Wlodzimierz Gut

Szymon Fila

Agnieszka Trzcinska

National Institute of Hygiene, Warsaw, Poland

Anna Roszkowiak

Military Institute of Hygiene and Epidemiology, Pulawy, Poland

Agnieszka E. Laudy

Medical University of Warsaw

Teresa Wernik

University Hospital for Children, Warsaw, Poland

Ewa Mikulak

Aleksandra Gliniewicz

Hanna Stypulkowska-Misiurewicz

National Institute of Hygiene, Warsaw, Poland

The aim of this study was to characterise the 34 Enterobacter cloacae strains isolated from different places in a hospital, either from patients who were infected or colonised and from the body surface of German cockroaches (Blatella germanica) caught in hospitals. A number of factors were determined: the ability of E. cloacae strains to adhere to the HEp-2 cell line, their susceptibility to drugs, the activity of disinfectant agents to planktonic cells and effectiveness of those disinfectants to bacteria that are sessile on catheters as well as the Enterobacterial Repetitive Intergenic Consensus – Polymerase Chain Reaction (ERIC-PCR) profile of the strains. On the basis of a statistical analysis of all the phenotypic results we were able to distinguish a few clusters. The majority of them consisted of strains isolated from colonisation and infection of patients. Some clusters consisted of E. cloacae from all the test sites. Some strains showed similarities of their phenotypic properties as well as the ERIC-PCR profile. Epidemiological studies are continuing.

Key Words: Enterobacter cloacae • Virulence • Hospital environment

References

• Jiang X, Ni Y, Jiang Y, Yuan F, Han L, Li M, Liu H, Yang L, Lu Y: Outbreak of infection caused by Enterobacter cloacae the novel VEB-3 beta-lactamase in China . J Clin Microbiol 2005;43: 826–831 .[Abstract/Free Full Text]
• Ma L, Chang FY, Fung CP, Chen TL, Lin JC, Lu PL, Huang LY, Chang JC, Siu LK: Variety of TEM-, SHV-, and CTX-M-type beta-lactamases present in recent clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae from Taiwan . Microb Drug Resist 2005;11: 31–39 .[CrossRef][Medline] [Order article via Infotrieve]
• Munday CJ, Whitehead GM, Todd NJ, Campbell M, Hawkey PM: Predominance and genetic diversity of community and hospitalacquired CTX-M extended-spectrum beta-lactamases in York, UK . J Antimicrob Chemothr 2004;54: 628–633 .
• Saverino D, Militenda-Floriani F, Medeiros AA: Effect of cefoxitin and clindamycin on selection of derepressed mutants in Enterobacter cloacae . New Microbial 2004;27: 249–253 .
• Pietrzyk A, Wójkowska-Mach J, Bulanda M, Heczko PB: Hospital-acquired pneumonia: analyses of frequency and aetiology in Polish hospitals during 1998 . Przeg Epidemiol 2000;54: 259–269 .
• Staszków E, Wójkowska-Mach J, Kuthan R, Bulanda M, Heczko PB: Dominant etiological factors of urinary tract infections in various hospital departments and their resistance to chemotherapeutic agents . Przeg Epidemiol 2000;54: 271–279 .
• Livrelli V, De Champs C, Di Martino P, Darfeuille-Michaud A, Forestier C, Joly B: Adhesive properties and antibiotic resistance of Klebsiella, Enterobacter, and Serratia clinical isolates involved in nosocomial infections . J Clin Microbiol 1996;34: 1963–1969 .[Abstract]
• Talon D, Menget P, Thouverez M, Thiriez G, Gbaguidi Haore H, Fromentin C, Muller A, Bertrand X: Emergence of Enterobacter cloacae as a common pathogen in neonatal units: pulsed field gel electrophoresis analysis . J Hosp Infect 2004;57: 119–125 .[CrossRef][Medline] [Order article via Infotrieve]
• Wang FD, Lin ML, Liu CY: Bacteraemia in Patients with hematological malignancies . Chemotherapy 2005;51: 147–153 .[Medline] [Order article via Infotrieve]
• Gliniewicz A, Czajka E, Laudy AE, Kochman M, Grzegorzak K, Ziólkowska K, Sawicka B, Stypulkowska Misiurewicz H, Pancer K: German cockroaches (Blatella germanicaL.) as a potential source of pathogens causing nosocomial infections . Indoor Built Environ 2003;12: 55–60 .
• Paciorek J: Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea . J Med Microbiol 2002;51: 548–556 .[Abstract/Free Full Text]
• Rivera IG, Chowdhury MAR, Huq A, Jacobs D, Martins MT, Colwell RR: Enterobacterial repetitive intergenic consensus sequences and the PCR to generate fingerprints of genomic DNAs from Vibrio choleraeO1, O139, and non-O1 strains . Appl Environ Microbiol 1995;61: 2898–2904 .[Abstract]
• Kac G, Podglajen I, Vaupre S, Colardell N, Buu-Hof A, Gutman L: Molecular epidemiology-of extended-spectrum beta-lactamseproducing Enterobacteriaceae isolated from environmental and clinical specimens in a cardiac surgery intensive care unit . Infect Control Hosp Epidemiol 2004;25: 852–855 .[Medline] [Order article via Infotrieve]

Indoor and Built Environment, Vol. 15, No. 1, 99-104 (2006)
DOI: 10.1177/1420326X06062372


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Pancer, K. Articles by Stypulkowska-Misiurewicz, H. Search for Related Content Social Bookmarking                         What's this?